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rabbit polyclonal anti-insulin receptor (ir)  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal anti-insulin receptor (ir)
    Rabbit Polyclonal Anti Insulin Receptor (Ir), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-insulin+receptor+(ir)/anti+ir/pm35793654-699-2-11
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-insulin receptor (ir) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Thyroid Hormone Supplementation Restores Cognitive Deficit, Insulin Signaling, and Neuroinflammation in the Hippocampus of a Sporadic Alzheimer’s-like Disease Rat Model
    Article Snippet: A Bio-Rad semi-dry Trans-Blot cell system (Hercules, CA, USA) was then used to electrotransfer the proteins to nitrocellulose membranes. .. The membranes were then blocked for 2 h at room temperature with PBS containing 0.05% Tween-20 (TTBS) and 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with the following specific primary antibodies: rabbit polyclonal anti-insulin receptor (IR, 1:1000; Cat# Sc-711, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit monoclonal anti-Akt (1:2000; Cat# 4691, Cell Signalling Technology, Danvers, MA, USA), rabbit monoclonal anti-phospho-Akt (Ser473) (1:1000; Cat# 4060, Cell Signalling Technology, Danvers, MA, USA), mouse monoclonal anti-GSK3α/β (1:1000; Cat# Sc-56913, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti phospho-GSK3α/β (Ser21/9) (1:1000; Cat# 9331, Cell Signalling Technology, Danvers, MA, USA), rabbit monoclonal anti-Bcl2 (1:1000; Cat# 3498, Cell Signalling Technology, Danvers, MA, USA), rabbit Polyclonal anti Bax (1:1000; Cat# 2772, Cell Signalling Technology, Danvers, MA, USA), mouse monoclonal anti- GFAP (1:1000; Cat# G-3893, Sigma-Aldrich Chemie GmbH, Schnelldorf, Germany), rabbit Polyclonal anti-Tumor Necrosis Factor-α (TNF-α, 1:1000; Cat# ab6671, Abcam, Cambridge, MA, USA), mouse monoclonal anti-Interleukin-6 (IL-6, 1:500; Cat# ab9324, Abcam, Cambridge, MA, USA), and rabbit polyclonal anti-Monocarboxylate Transporter 8 (MCT8, 1:1000; Cat# ab192828, Abcam, Cambridge, MA, USA). .. In all of the experiments, a mouse monoclonal anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 1:1000; Cat# 51332, Cell Signalling Technology, Danvers, MA, USA) was used as a loading control.

    other:

    Article Title: Hierarchical glucocorticoid-endocannabinoid interplay regulates the activation of the nucleus accumbens by insulin
    Article Snippet: Proteins were then transferred into nitrocellulose membranes (Panreac Química, Barcelona, España) and probed with goat polyclonal anti-CB 1 R C-terminal antibody ( Rozenfeld and Levi, 2008 ; Bitencourt et al., 2015 ) and affinity purified rabbit polyclonal anti-InsR (Santa Cruz Biotechnology, sc-711), diluted in blocking solution (Tris-buffered saline supplemented with 0.1% Tween-20 and 5% milk).


    Article Title: Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells
    Article Snippet: Primary antibodies used for the western blotting were as follows: rabbit polyclonal anti-phospho-IGF-1R β Tyr1131/IR- β Tyr1146 (number 3021, Cell Signaling), rabbit polyclonal anti-IGF-IR β (sc-713, Santa Cruz Biotechnology), rabbit polyclonal anti-phospho-Shc (number 2434, Cell Signaling), rabbit polyclonal anti-Shc (sc-288, Santa Cruz), rabbit polyclonal anti-ER α (sc-7207, Santa Cruz), mouse monoclonal anti-phospho-ER α Ser118 (number 2511, Cell Signaling), rabbit polyclonal anti-MAPKp44/42, and rabbit polyclonal anti-phospho-MAPKp44/42 (number 9201 and number 9101, Cell Signaling).

    Article Title: HMGA1 deficiency: a pathogenic link between tau pathology and insulin resistance.
    Article Snippet: Department of Health Sciences, University of Catanzaro “Magna Græcia”, Catanzaro, Italy Department of Medical and Surgical Sciences, University of Catanzaro “Magna Græcia”, Catanzaro, Italy Department of Molecular Medicine and Medical Biotechnology, University of Naples “Federico II”, Naples, Italy Department of Experimental and Clinical Medicine, University of Catanzaro “Magna Græcia”, Catanzaro, Italy Interdepartmental Centre of Veterinary Radiology, University of Naples “Federico II”, Naples, Italy University of Lille, Inserm, CHU-Lille, Lille Neuroscience & Cognition, UMR-S1172, Team Alzheimer & Tauopathies, F-59000, Lille, France Department of Physiology and Pharmacology, University of Rome “Sapienza”, Rome, Italy Institute of Experimental Endocrinology and Oncology, CNR, Naples, Italy IRCCS Neuromed, Pozzilli, Italy

    Article Title: HMGA1 deficiency: a pathogenic link between tau pathology and insulin resistance
    Article Snippet: For immunofluorescence assays, monocytes were fixed in glutaraldehyde, permeabilized with 0.1% Triton X-100, and incubated with primary polyclonal rabbit anti-human INSR antibody (N-20, α chain, sc-710; Santa Cruz Biotechnology; RRID: AB_631106 ) for 2 h at room temperature.

    Staining:

    Article Title: Characterizing Adrenergic Regulation of Glucose Transporter 4-Mediated Glucose Uptake and Metabolism in the Heart
    Article Snippet: .. After transfer, membranes were stained with anti-GLUT4 antibody (1:1000, ab654; Abcam), anti-insulin receptor β antibody (C-19, rabbit polyclonal immunoglobulin G [IgG], SC-711; Santa Cruz Biotechnology), or anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:10000, MAB374; Millipore) antibodies. .. Primary antibodies were detected either with IRDye 800 CW Goat anti-Rabbit IgG secondary antibody (1:10000, #926-32211; Licor) or IRDye 800CW Goat anti-Mouse IgG secondary antibody (1:10000, #926-32210; Licor) using ChemiDoc Imaging System (Bio-Rad).



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    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, <t>IRS1,</t> p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B
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    Santa Cruz Biotechnology rabbit polyclonal anti insulin receptor ir
    KEY RESOURCES TABLE
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    Image Search Results


    Reaction of polyclonal IR-A, GAD-65, and GAD-67 antibodies with different dietary proteins.

    Journal: Journal of Diabetes Research

    Article Title: Detection of Islet Cell Immune Reactivity with Low Glycemic Index Foods: Is This a Concern for Type 1 Diabetes?

    doi: 10.1155/2017/4124967

    Figure Lengend Snippet: Reaction of polyclonal IR-A, GAD-65, and GAD-67 antibodies with different dietary proteins.

    Article Snippet: Mouse monoclonal anti-insulin antibodies, affinity-purified rabbit polyclonal insulin receptor alpha (IR-A) antibodies, mouse monoclonal insulin receptor beta (IR-B) antibodies, affinity-purified rabbit polyclonal GAD-65 antibodies, and affinity-purified rabbit polyclonal GAD-67 antibodies were purchased from Abcam (Cambridge, MA, USA).

    Techniques:

    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

    doi: 10.1186/s40104-019-0347-4

    Figure Lengend Snippet: Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-protein kinase B (AKT) (#9272S; 1:1,000 dilution), rabbit monoclonal anti-phospho(p)-AKT (Ser473) (#9272; 1:2,000 dilution), rabbit monoclonal anti-p-AKT (Thr308) (D25E6; 1:1,000 dilution), mouse monoclonal anti-INSR (ab69508; 1:1,000 dilution), rabbit polyclonal anti-insulin receptor substrate 1 (IRS1) (#2382; 1:1,000 dilution), rabbit polyclonal anti-p-IRS1 (#2381; 1:1,000 dilution) (Cell Signaling Technology, Inc., Danvers, MA, USA), and mouse anti-β-actin Mab (66009–1-Ig; 1:1,000 dilution; Proteintech Group Inc., Chicago, IL, USA).

    Techniques: Western Blot, Control, Software

    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Inflammation Improves Glucose Homeostasis Through IKKβ-XBP1s Interaction

    doi: 10.1016/j.cell.2016.10.015

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit polyclonal anti-insulin receptor (IR) , Santa Cruz Biotechnology , Cat#sc-711.

    Techniques: Recombinant, Plasmid Preparation, Expressing, Sequencing, Software